Coding

Part:BBa_K4908000

Designed by: Yang Hanqiu   Group: iGEM23_SubCat-Shanghai   (2023-07-27)


MS18

MS18

Profile

Name: MS18

Base Pairs: 495 bp

Origin: Vibrio parahaemolyticus

Properties: Sequences of genes capable of encoding functional proteins

Usage and Biology

The gene fragment we used comes from the tail fiber protein (TFP) MS18 of Vibrio parahaemolyticus phage VPMS1. Vibrio parahaemolyticus is a marine bacterium that mainly comes from seafood such as fish, shrimp, crab, shellfish, and seaweed. Consumption of food containing this bacterium can lead to food poisoning, also known as halophilic food poisoning. Clinically, acute abdominal pain, vomiting, diarrhea, and watery stools are the main symptoms[1]. This gene is a characteristic fragment of Vibrio parahaemolyticus.

Reference

[1] Ramírez-Orozco Martín, Serrano-Pinto Vania, Ochoa-Álvarez Norma, Makarov Roman, Martínez-Díaz Sergio F. Genome sequence analysis of the Vibrio parahaemolyticus lytic bacteriophage VPMS1.[J]. Archives of virology, 2013, 158(11)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


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